Okay, honestly, theoretically, you aren't supposed to be able to tell the difference.
But, with all the different encoders and all the different equipment people have, not to mention whether or not you know what's going on in the music, most times (if not all), when you download stuff, there will be a
big difference in the quality between a 128 and 320 rip.
About your itunes rip, back in the day, I ripped my (miss)understood CD in 128 using itunes, when I ripped it again in 320 later, there was very very little difference...so much that, I'm not even sure there was a difference. the 128 may not have been as loud...but seriously, it was really negligible. So, it really just depends.
I personally find it a little weird the site has them both in 128 and 320 and charges more for the 320...seems like they're just trying to squeeze munny out of you. That being said, they could have purposely made the 128's really low quality or something. Or there could be very little difference...I think you're kinda on your own with this one...unless someone here has personal experience with the site. Sorry